IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Viral hypothesis in sporadic amyotrophic lateral sclerosis (sALS)”
Autor/es:
BETTINI M; REGUEIRO M; GARGIULO MONACHELLI GM; RODRIGUEZ G; REY RC; SICA REP; PARADA V; FREIRE MC; EILIAN E; MARTINEZ PERALTA L
Reunión:
Congreso; 63rd Annual Meeting of the American Academy of Neurology.; 2011
Resumen:
Background and objectives: Some viruses have been proposed as probable environmental factors for sALS, including adenovirus, enterovirus, poliovirus, HTLV and more recently cases of motor neuron disease have been reported in patients with HIV. The aim of the study is to explore the hypothesis of a viral environmental factor in sALS by PCR detection of viral genome in muscle biopsies in patients and controls. Methods: Samples were obtained from the biceps and deltoid muscles of sALS patients and controls, through surgical or percutaneous biopsy. They were homogenized and nucleic acids were extracted using a commercial kit for column purification of DNA and RNA (QIAGEN, Austin Texas, USA). We performed Nested-PCR with specific primers for each virus tested. For enterovirus, the primers amplified a conserved region, while for herpesvirus we developed a multiplex testing HSV, VZV and CMV. Finally, the amplification products and their controls were run on agarose gels and visualized with LUV. Results: The samples corresponded to 10 patients with definite diagnosis of ALS, 3 were women and 7 men, age at diagnosis was 55,5  7.8 (42-69) years [mean  SD (range)], and time onset diagnosis was 14 ± 8.8 (3-29) months. Control patients were 7 (4 with muscle disease and 3 healthy), of which 4 were women and 3 men. Age in this group was 45.2  13 years (23-65). PCR assays demonstrated uniformly negative results between the 2 groups. Conclusion: Viral genome was not detected in muscle tissue in either sALS or controls. To our knowledge, this is the first study to explore this hypothesis at this level and with various viral agents.