Presencia de miembros de la familia Glipr1 en el tracto reproductor masculino del ratón

ERNESTO JI; CALB DE; VISCONTI PE; COHEN DJ; CUASNICU PS

Buenos Aires

Jornada; XII Jornadas Multidisciplinarias de la Sociedad Argentina de Biología; 2010

Sociedad Argentina de Biología

The analysis of plasmatic membrane lipid RAFTs of mouse spermreveals the presence of GLIPR1l1 and GLIPR1l2 proteins which, as CRISP proteins, are members of the CAP superfamily. Considering the CRISP participation in different events of fertilization, in the present work we cloned and characterized Glipr1l1 and Glipr1l2 as a first step towards the study of their potential role in the fertilization process. rt-PCR studies revealed the testicular expression of Glipr1l1 and both the testicular and epididymal expression of Glipr1l2. Semi-quantitative rt-PCR showed a decrease in mRNA expression from birth to day 7, and a gradual increase thereafter reaching maximum levels at 60 days. The positive clones were subjected to IPTG induction and the detected recombinant proteins were then successfully purified. The use of specific antibodies against the human homologous of Glipr1l1 and Glipr1l2 indicated the presence of both proteins in fresh human sperm as well as their permanence after capacitation, supporting their possible participation in subsequent sperm-oocyte interaction. Altogether, the results obtained in mouse and human will allow the study of the potential role of GLIPR1 proteins in the maturation, capacitation and fertilization processes.