Las respuestas a kisspeptina están alteradas en explantos hipotalámicos de ratones adultos GABAB1KO

DI GIORGIO N; CATALANO P; BETTLER B; LIBERTUN C; LUX-LANTOS V

Buenos Aires

Jornada; XII Jornadas Anuales de la Sociedad Argentina de Biología (SAB); 2010

SAB

Adult GABAB1KO mice (KO) show a deregulation of GnRH compared to wild-types (WT), especially in females (AJP-EM.2010;298(3):E683-96). We studied the responses to Kisspeptin in GnRH secretion (RIA) and expression (qRT-PCR) and GAD67 (GAD) expression in hypothalamic explants (HTE) of adult mice (both sexes and genotypes), incubated with Kiss-10 (1.10-8M) or buffer (control) for 150 min. Kiss-10 increased GnRH secretion in all groups (interactions ns, treatment p<0.001). However, in the Kiss-10 group, GnRH secretion was lower in KO compared to WT males, without genotype differences in females. GnRH expression was affected by sex, genotype and treatment (triple interaction p<0.03). In basal conditions, KO males had lower GnRH expression than KO females and WT males, without sexual differences in WTs. Kiss-10 increased GnRH expression only in KO males, without differences between Kiss-10 groups. GnRH expression was higher in basal than Kiss-10 females. GAD expression was affected by sex, genotype and treatment (triple interaction p<0.05). Basal GAD was higher in males than females and higher in KOs than WTs. GAD in Kiss-10 males was similar to basal males, keeping their genotype differences. GAD increased in Kiss-10 WT females compared to basal females, without differences in KO females. Responses to Kisspeptin are sex-specifically altered in HTE of KO mice. (CONICET-ANPCYT-UBA)