IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Neurotrophic factor and neurogenesis in oestradiol neuroprotection of the hippocampus of hypertensive rats
Autor/es:
PIETRANERA, LUCIANA; LIMA, ANALÍA; ROIG, PAULINA; DE NICOLA, ALEJANDRO
Revista:
JOURNAL OF NEUROENDOCRINOLOGY.
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2010 vol. 22 p. 1082 - 1092
ISSN:
0953-8194
Resumen:
The hippocampus of spontaneously hypertensive rats (SHR) and deoxycorticosterone (DOCA)-salt hypertensive rats shows decreased cell proliferation, astrogliosis and reduced number of hilar cells. These defects are corrected after a 2 week administration with 17 b-oestradiol. The present work investigated if 17 b-oestradiol treatment of SHR and of hypertensive DOCA-salt rats modulated the expression of brain-derived neurotrophic factor (BDNF), a neurotrophin involved in hippocampal neurogenesis. The neurogenic response to 17b-oestradiol was simultaneously determined by counting the number of doublecortin-immunopositive neuroblasts in the subgranular layer of the dentate gyrus. Both hypertensive models showed decreased expression of BDNF mRNA in the granular zone of the dentate gyrus, without changes in CA1 or CA3 pyramidal cell layers, decreased BDNF protein levels in whole hippocampal tissue and also low density of doublecortin-positive neuroblasts in the subgranule layer of the dentate gyrus. After sc implantation of a single 17 b-oestradiol pellet for 2 weeks, BDNF mRNA in the dentate gyrus, BDNF protein in whole hippocampus and doublecortin immunopositive neuroblasts were significantly raised in both SHR and DOCA-salt-treated rats. These results indicate that: a) low BDNF expression and deficient neural progenitors distinguish the hippocampus of SHR and DOCA-salt hypertensive rats; b) 17 b-oestradiol was able to recover these biologically important functions in the hippocampus of hypertensive animals.