Effects of progesterona on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury

LABOMBARDA F; GONZALEZ S; LIMA A; ROIG P; GUENNOUN R; SCHUMACHER M; DE NICOLA A

GLIA

Año: 2009 vol. 57 p. 884 - 897

0894-1491

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margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Courier New"; mso-fareast-font-family:Batang; mso-ansi-language:EN-GB; mso-fareast-language:ES;} p {margin-right:0cm; mso-margin-top-alt:auto; mso-margin-bottom-alt:auto; margin-left:0cm; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:Batang; mso-ansi-language:ES; mso-fareast-language:ES;} pre {margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; tab-stops:45.8pt 91.6pt 137.4pt 183.2pt 229.0pt 274.8pt 320.6pt 366.4pt 412.2pt 458.0pt 503.8pt 549.6pt 595.4pt 641.2pt 687.0pt 732.8pt; font-size:10.0pt; font-family:"Courier New"; mso-fareast-font-family:Batang; mso-ansi-language:ES; mso-fareast-language:ES;} p.Default, li.Default, div.Default {mso-style-name:Default; mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; mso-layout-grid-align:none; text-autospace:none; font-size:12.0pt; font-family:Arial; mso-fareast-font-family:Batang; color:black; mso-ansi-language:ES; mso-fareast-language:ES;} span.volume {mso-style-name:volume;} span.issue {mso-style-name:issue;} span.pages {mso-style-name:pages;} span.ti {mso-style-name:ti;} span.featuredlinkouts {mso-style-name:featured_linkouts;} span.linkbar {mso-style-name:linkbar;} span.pseudotab2 {mso-style-name:pseudotab2;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Progesterone is emerging as a myelinizing factor for central nervous system injury. Successful remyelination requires proliferation and differentiation of oligodendrocyte precursor cells (OPC) into myelinating oligodendrocytes (OL), but this process is incomplete following injury. To study progesterone actions on remyelination, we administered progesterone (16 mg/kg/day) to rats with complete spinal cord injury. Rats were euthanized 3 or 21 days after steroid treatment. Short progesterone treatment: a) increased the number of OPC without effect on the injury-induced reduction of mature OL; b) increased mRNA and protein expression for the myelin basic protein (MBP) without effects on proteolipid protein (PLP) or myelin oligodendrocyte glycoprotein (MOG) and c) increased the mRNA for Olig2 and Nkx2.2 transcription factors involved in specification and differentiation of the OL lineage. Furthermore, long progesterone treatment: a) reduced OPC with a concomitant increase of OL; b) promoted differentiation of cells that incorporated bromodeoxyuridine, early after injury, into mature OL; c) increased mRNA and protein expression of PLP without effects on MBP or MOG and d) increased mRNA for the Olig1 transcription factor involved in myelin repair. These results suggest that early progesterone treatment enhanced the density of OPC and induced their differentiation into mature OL by increasing the expression of Olig2 and Nkx2.2. Twenty one days after injury, progesterone favors remyelination by increasing Olig1 (involved in repair of demyelinated lesions), PLP expression and enhancing OL maturation. Thus, progesterone effects on oligodendrogenesis and myelin proteins may constitute fundamental steps for repairing traumatic injury inflicted to the spinal cord