Interferon-ã inhibits metalloproteinase activity and cytotrophoblast cell migration

FONTANA VANINA; SANCHEZ MELISA; CEBRAL ELISA; CALVO JUAN CARLOS

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: New Jersey; Año: 2010 vol. 64 p. 20 - 26

1046-7408

The establishment of a successful pregnancy relies on a complex fetal–mother communication that starts with the embryo adhering and invad-ing the endometrium. This invasion requires remodeling of extracellularmatrix, performed by metalloproteinases. Cytokines, such as c-interferon(c-IFN), play a role in implantation and could have profound effects onthe success of pregnancy. Here, we demonstrate that c-IFN inhibits gela-tinase activity from matrix metalloproteinase (MMP)-2 and MMP-9 in adose-dependent manner, reducing the secretion of leptin (not becauseof a general inhibition on protein synthesis) and impairs cell migrationon Matrigel, using JEG-3 cell line as cytotrophoblastic model. This corre-lates with previous reports from our laboratory indicating that c-IFN isdeleterious for mouse embryo outgrowth.