IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
N-acetylcysteine inhibits kinase phosphorylation during 3T3-L1 adipocyte differentiation
Autor/es:
SOTO, DANIELA; CALVO, JUAN C.; GOMEZ-SERRANO, MARÍA; SOTO, DANIELA; PERAL, BELÉN; CALVO, JUAN C.; GOMEZ-SERRANO, MARÍA; PERAL, BELÉN; PIERALISI, AZUL; GUERRA, LILIANA N.; PIERALISI, AZUL; GUERRA, LILIANA N.
Revista:
REDOX REPORT
Editorial:
MANEY PUBLISHING
Referencias:
Año: 2017 vol. 22 p. 265 - 271
ISSN:
1351-0002
Resumen:
Objectives: Reports investigating the effects of antioxidants on obesity have provided contradictory results. We have previously demonstrated that treatment with the antioxidant N-acetylcysteine (NAC) inhibits cellular triglyceride (Tg) accumulation as well as total cellular monoamine oxidase A (MAOA) expression in 3T3-L1 mature adipocytes (Calzadilla et al., Redox Rep. 2013;210?218). Here we analyzed the role of NAC on adipogenic differentiation pathway. Methods: Assays were conducted using 3T3-L1 preadipocytes (undifferentiated cells: CC), which are capable of differentiating into mature adipocytes (differentiated cells: DC). We studied the effects of different doses of NAC (0.01 or 1 mM) on DC, to evaluate cellular expression of phospho-JNK½ (pJNK½), phospho-ERK½ (pERK½) and, mitochondrial expression of citrate synthase, fumarate hydratase and MAOA. Results: Following the differentiation of preadipocytes, an increase in the expression levels of pJNK½ and pERK½ was observed, together with mitotic clonal expansion (MCE). We found that both doses of NAC decreased the expression of pJNK½ and pERK½. Consistent with these results, NAC significantly inhibited MCE and modified the expression of different mitochondrial proteins. Discussion: Our results suggested that NAC could inhibit Tg and mitochondrial protein expression by preventing both MCE and kinase phosphorylation.