IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
ERα and GnRH co-localize in the hypothalamic neurons of the South American plains vizcacha, Lagostomus maximus (Rodentia, Caviomorpha)
Autor/es:
INSERRA, PABLO IGNACIO FELIPE; CHARIF, SANTIAGO ELÍAS; DI GIORGIO, NOELIA PAULA; SAUCEDO, LUCÍA; SCHMIDT, ALEJANDRO RAÚL; FRAUNHOFFER, NICOLAS; HALPERIN, JULIA; GARIBOLDI, MARÍA CONSTANZA; LEOPARDO, NOELIA PAOLA; LUX-LANTOS, VICTORIA; GONZALEZ, CANDELA ROCÍO; VITULLO, ALFREDO DANIEL; DORFMAN, VERÓNICA BERTA; INSERRA, PABLO IGNACIO FELIPE; CHARIF, SANTIAGO ELÍAS; DI GIORGIO, NOELIA PAULA; SAUCEDO, LUCÍA; SCHMIDT, ALEJANDRO RAÚL; FRAUNHOFFER, NICOLAS; HALPERIN, JULIA; GARIBOLDI, MARÍA CONSTANZA; LEOPARDO, NOELIA PAOLA; LUX-LANTOS, VICTORIA; GONZALEZ, CANDELA ROCÍO; VITULLO, ALFREDO DANIEL; DORFMAN, VERÓNICA BERTA
Revista:
JOURNAL OF MOLECULAR HISTOLOGY
Editorial:
SPRINGER
Referencias:
Año: 2017 vol. 48 p. 259 - 273
ISSN:
1567-2379
Resumen:
Gonadotropin-releasing hormone (GnRH) is the key regulator of the hypothalamic-pituitary-gonadal axis. Estradiol (E2) affects GnRH synthesis and delivery. Hypothalamic estrogen receptors (ER) modulate GnRH expression acting as transcription factors. The South American plains vizcacha, Lagostomus maximus, is able to ovulate up to 800 oocytes per reproductive cycle, and shows continuous folliculogenesis with pre-ovulatory follicle formation and an ovulatory event at mid-gestation. The aim of this work was to analyze the hypothalamic expression of ER in the vizcacha at different gestational time-points, and its relationship with GnRH expression, serum luteinizing hormone (LH) and E2. The hormonal pattern of mid-gestating vizcachas was comparable to ovulating-females with significant increases in GnRH, LH and E2. Hypothalamic protein and mRNA expression of ERα varied during pregnancy with a significant increase at mid-gestation whereas ERβ mRNA expression did not show significant variations. Hypothalamic immunolocalization of ERα was observed in neurons of the diagonal band of Brocca, medial preoptic area (mPOA), periventricular, suprachiasmatic, supraoptic (SON), ventromedial, and arcuate nuclei, and medial eminence, with a similar distribution throughout gestation. In addition, all GnRH neurons of the mPOA and SON showed ERα expression with no differences across the reproductive status. The correlation between GnRH and ERα at mid-gestation, and their co-localization in the hypothalamic neurons of the vizcacha, provides novel information compared with other mammals suggesting a direct action of estrogen as part of a differential reproductive strategy to assure GnRH synthesis during pregnancy.