IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa.
Autor/es:
CALAMERA JC; BUFFONE MG; DONCEL GF; BRUGO-OLMEDO S; DE VINCENTIIS S; CALAMERA MM; STOREY BT; ALVAREZ JG
Revista:
FERTILITY AND STERILITY
Editorial:
Elsevier Science
Referencias:
Lugar: San Diego, CA. USA; Año: 2010 vol. 93 p. 789 - 794
ISSN:
0015-0282
Resumen:
OBJECTIVE: To investigate the effects of thawing temperature on sperm function after cryopreservation. The technical aspects of sperm cryopreservation have significantly improved over the last few decades. However, a standard protocol designed to optimize sperm motility recovery after thawing has not yet been established. DESIGN: Prospective study. SETTING: Private infertility institute and university-based research laboratory. PATIENT(S): Eighty consenting normozoospermic patients consulting for infertility. INTERVENTION(S): Spermatozoa from donor semen samples were thawed at different temperatures. MAIN OUTCOME MEASURE(S): Sperm motility, viability, adenosine-5´-triphosphate (ATP) content, acrosomal status, and DNA integrity were evaluated as a function of thawing temperature in cryopreserved human sperm samples. RESULT(S): Thawing at 40 degrees C resulted in a statistically significant increase in sperm motility recovery compared with thawing at temperatures between 20 degrees C and 37 degrees C. There were no statistically significant differences in sperm viability, acrosomal status, ATP content, and DNA integrity after thawing at 40 degrees C compared with thawing at temperatures between 20 degrees C and 37 degrees C. CONCLUSION(S): Sperm thawing at 40 degrees C could be safely used to improve motility recovery after sperm cryopreservation.