IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Immunological behaviour of human cysteine-rich secretory protein 1 (hCRISP1) in primates: prospects for immunocontraception
Autor/es:
ELLERMAN DA; COHEN DJ; WEIGEL MUÑOZ M; DA ROS VG; ERNESTO JI; TOLLNER T; CUASNICU PS
Revista:
FERTILITY AND STERILITY
Editorial:
ELSEVIER
Referencias:
Lugar: New York, USA; Año: 2009
ISSN:
0015-0282
Resumen:
ABSTRACT Objective: To evaluate the immunological behaviour of hCRISP1, a human sperm epididymal protein involved in fertilization, as an approach to establish itsthe immunocontraceptive potential. Design: In vivo study in a non-human primate model. Setting: Animal care facility of an academic research center. Animal(s): Adult (6-15 years old) male and female cynomolgus macaques (Macaca fascicularis) distributed into three groups. Intervention(s): Animals received 4 injections (intramuscularly) of recombinant hCRISP1, recombinant monkey CRISP1 (mkCRISP1), or MBP (maltose binding protein). Blood and semen samples were obtained before and after immunization. Main Outcome Measure(s): Anti-hCRISP1 and anti-mkCRISP1 levels in sera and seminal plasma were evaluated by enzyme-linked immunosorbent assay (ELISA). The specificity of the immune response was evaluated by Western Blot and binding of the antibodies to sperm by immunofluorescence. Results(s): hCRISP1 and mkCRISP1 raised an immune response that increased as a function of time and specifically recognizes mkCRISP1 in sperm extracts. Sperm number, motility and morphology were not affected by immunization. The presence of both specific antibodies in seminal plasma and a fluorescent labeling in sperm exposed only to second antibody indicated the ability of the anti-hCRISP1 antibodies to both enter into the male reproductive tract and bind to the cells in vivo. 5 Conclusion (s): These results support the potential involvement of anti-hCRISP1 antibodies in human immunoinfertility and hCRISP1 as a likely candidate for immunocontraception. Keywords: contraception, epididymis, sperm, immunoinfertility.