IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Immunological behaviour of human cysteine-rich secretory protein 1 (hCRISP1) in primates: prospects for immunocontraception
Autor/es:
ELLERMAN DA; COHEN DJ; WEIGEL MUÑOZ M; DA ROS VG; ERNESTO JI; TOLLNER T; CUASNICU PS
Revista:
FERTILITY AND STERILITY
Editorial:
ELSEVIER
Referencias:
Lugar: New York, USA; Año: 2009
ISSN:
0015-0282
Resumen:
ABSTRACT
Objective: To evaluate the immunological behaviour of hCRISP1, a
human sperm epididymal protein involved in fertilization, as an
approach to establish itsthe immunocontraceptive potential.
Design: In vivo study in a non-human primate model.
Setting: Animal care facility of an academic research center.
Animal(s): Adult (6-15 years old) male and female cynomolgus macaques (Macaca
fascicularis) distributed into three groups.
Intervention(s): Animals received 4 injections (intramuscularly) of recombinant hCRISP1,
recombinant monkey CRISP1 (mkCRISP1), or MBP (maltose binding protein). Blood and
semen samples were obtained before and after immunization.
Main Outcome Measure(s): Anti-hCRISP1 and anti-mkCRISP1 levels in sera and seminal
plasma were evaluated by enzyme-linked immunosorbent assay (ELISA). The specificity of
the immune response was evaluated by Western Blot and binding of the antibodies to sperm
by immunofluorescence.
Results(s): hCRISP1 and mkCRISP1 raised an immune response that increased as a function
of time and specifically recognizes mkCRISP1 in sperm extracts. Sperm number, motility
and morphology were not affected by immunization. The presence of both specific
antibodies in seminal plasma and a fluorescent labeling in sperm exposed only to second
antibody indicated the ability of the anti-hCRISP1 antibodies to both enter into the male
reproductive tract and bind to the cells in vivo.
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Conclusion (s): These results support the potential involvement of anti-hCRISP1 antibodies
in human immunoinfertility and hCRISP1 as a likely candidate for immunocontraception.
Keywords: contraception, epididymis, sperm, immunoinfertility.