IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Differential gene expression in the rat hippocampus during learning of an operant conditioning task
Autor/es:
RAPANELLI, M., FRICK, L.R., ZANUTTO, B.S.
Revista:
NEUROSCIENCE
Editorial:
Elsevier
Referencias:
Lugar: Estados Unidos; Año: 2009 vol. 163 p. 1031 - 1038
ISSN:
0306-4522
Resumen:
AbstractChanges in transcription levels of brain-derived
neurotrophic factor (BDNF), cyclic adenosine monophosphate
(cAMP) response element binding (CREB), Synapsin I,
Ca2/calmodulin-dependent protein kinase II (CamKII), activity-
regulated cytoskeleton-associated protein (Arc), c-jun and
c-fos have been associated to several learning paradigms in
different brain areas. In this study, we measured mRNA expression
in the hippocampus by real time (RT)-PCR mRNA
levels of BDNF, CREB, Synapsin I, CamKII, Arc, c-jun and
c-fos, during learning and operant conditioning task. Experimental
groups were as follows: control (C, the animals never
left the bioterium), when the animals reached 5065% of the
expected response (Incompletely Trained, IT), when animals
reached 100% of the expected response with a latency time
lower than 5 s (Trained, Tr), Box Control of Incompletely
Trained (BCIT), animals spent the same time as the IT in the
operant conditioning box and Box Control of Trained (BCTr)
animals spent the same time as the Tr in the operant conditioning
box. All rats were killed at the same time by cervical
dislocation 15 min after training and hippocampi were removed
and processed. We found increments of mRNA levels
of most genes (BDNF, CREB, Synapsin I, Arc, c-jun and c-fos)
in IT and Tr groups compared to their box controls, but
increments in Tr were smaller compared with IT. These results
describe a differential gene expression in the rat hippocampus
when the animals are learning and when animals
have already learned. Taking together the results presented
herein with the known functions of these genes, we propose
a link between changes in gene expression in the hippocampus
and different degrees of cellular activation and plasticity
during learning of an operant conditioning task. © 2009 IBRO.
Published by Elsevier Ltd. All rights reserved.
Key words: hippocampus, real-time PCR, learning.
regulated cytoskeleton-associated protein (Arc), c-jun and
c-fos have been associated to several learning paradigms in
different brain areas. In this study, we measured mRNA expression
in the hippocampus by real time (RT)-PCR mRNA
levels of BDNF, CREB, Synapsin I, CamKII, Arc, c-jun and
c-fos, during learning and operant conditioning task. Experimental
groups were as follows: control (C, the animals never
left the bioterium), when the animals reached 5065% of the
expected response (Incompletely Trained, IT), when animals
reached 100% of the expected response with a latency time
lower than 5 s (Trained, Tr), Box Control of Incompletely
Trained (BCIT), animals spent the same time as the IT in the
operant conditioning box and Box Control of Trained (BCTr)
animals spent the same time as the Tr in the operant conditioning
box. All rats were killed at the same time by cervical
dislocation 15 min after training and hippocampi were removed
and processed. We found increments of mRNA levels
of most genes (BDNF, CREB, Synapsin I, Arc, c-jun and c-fos)
in IT and Tr groups compared to their box controls, but
increments in Tr were smaller compared with IT. These results
describe a differential gene expression in the rat hippocampus
when the animals are learning and when animals
have already learned. Taking together the results presented
herein with the known functions of these genes, we propose
a link between changes in gene expression in the hippocampus
and different degrees of cellular activation and plasticity
during learning of an operant conditioning task. © 2009 IBRO.
Published by Elsevier Ltd. All rights reserved.
Key words: hippocampus, real-time PCR, learning.
regulated cytoskeleton-associated protein (Arc), c-jun and
c-fos have been associated to several learning paradigms in
different brain areas. In this study, we measured mRNA expression
in the hippocampus by real time (RT)-PCR mRNA
levels of BDNF, CREB, Synapsin I, CamKII, Arc, c-jun and
c-fos, during learning and operant conditioning task. Experimental
groups were as follows: control (C, the animals never
left the bioterium), when the animals reached 5065% of the
expected response (Incompletely Trained, IT), when animals
reached 100% of the expected response with a latency time
lower than 5 s (Trained, Tr), Box Control of Incompletely
Trained (BCIT), animals spent the same time as the IT in the
operant conditioning box and Box Control of Trained (BCTr)
animals spent the same time as the Tr in the operant conditioning
box. All rats were killed at the same time by cervical
dislocation 15 min after training and hippocampi were removed
and processed. We found increments of mRNA levels
of most genes (BDNF, CREB, Synapsin I, Arc, c-jun and c-fos)
in IT and Tr groups compared to their box controls, but
increments in Tr were smaller compared with IT. These results
describe a differential gene expression in the rat hippocampus
when the animals are learning and when animals
have already learned. Taking together the results presented
herein with the known functions of these genes, we propose
a link between changes in gene expression in the hippocampus
and different degrees of cellular activation and plasticity
during learning of an operant conditioning task. © 2009 IBRO.
Published by Elsevier Ltd. All rights reserved.
Key words: hippocampus, real-time PCR, learning.
regulated cytoskeleton-associated protein (Arc), c-jun and
c-fos have been associated to several learning paradigms in
different brain areas. In this study, we measured mRNA expression
in the hippocampus by real time (RT)-PCR mRNA
levels of BDNF, CREB, Synapsin I, CamKII, Arc, c-jun and
c-fos, during learning and operant conditioning task. Experimental
groups were as follows: control (C, the animals never
left the bioterium), when the animals reached 5065% of the
expected response (Incompletely Trained, IT), when animals
reached 100% of the expected response with a latency time
lower than 5 s (Trained, Tr), Box Control of Incompletely
Trained (BCIT), animals spent the same time as the IT in the
operant conditioning box and Box Control of Trained (BCTr)
animals spent the same time as the Tr in the operant conditioning
box. All rats were killed at the same time by cervical
dislocation 15 min after training and hippocampi were removed
and processed. We found increments of mRNA levels
of most genes (BDNF, CREB, Synapsin I, Arc, c-jun and c-fos)
in IT and Tr groups compared to their box controls, but
increments in Tr were smaller compared with IT. These results
describe a differential gene expression in the rat hippocampus
when the animals are learning and when animals
have already learned. Taking together the results presented
herein with the known functions of these genes, we propose
a link between changes in gene expression in the hippocampus
and different degrees of cellular activation and plasticity
during learning of an operant conditioning task. © 2009 IBRO.
Published by Elsevier Ltd. All rights reserved.
Key words: hippocampus, real-time PCR, learning.
regulated cytoskeleton-associated protein (Arc), c-jun and
c-fos have been associated to several learning paradigms in
different brain areas. In this study, we measured mRNA expression
in the hippocampus by real time (RT)-PCR mRNA
levels of BDNF, CREB, Synapsin I, CamKII, Arc, c-jun and
c-fos, during learning and operant conditioning task. Experimental
groups were as follows: control (C, the animals never
left the bioterium), when the animals reached 5065% of the
expected response (Incompletely Trained, IT), when animals
reached 100% of the expected response with a latency time
lower than 5 s (Trained, Tr), Box Control of Incompletely
Trained (BCIT), animals spent the same time as the IT in the
operant conditioning box and Box Control of Trained (BCTr)
animals spent the same time as the Tr in the operant conditioning
box. All rats were killed at the same time by cervical
dislocation 15 min after training and hippocampi were removed
and processed. We found increments of mRNA levels
of most genes (BDNF, CREB, Synapsin I, Arc, c-jun and c-fos)
in IT and Tr groups compared to their box controls, but
increments in Tr were smaller compared with IT. These results
describe a differential gene expression in the rat hippocampus
when the animals are learning and when animals
have already learned. Taking together the results presented
herein with the known functions of these genes, we propose
a link between changes in gene expression in the hippocampus
and different degrees of cellular activation and plasticity
during learning of an operant conditioning task. © 2009 IBRO.
Published by Elsevier Ltd. All rights reserved.
Key words: hippocampus, real-time PCR, learning.
regulated cytoskeleton-associated protein (Arc), c-jun and
c-fos have been associated to several learning paradigms in
different brain areas. In this study, we measured mRNA expression
in the hippocampus by real time (RT)-PCR mRNA
levels of BDNF, CREB, Synapsin I, CamKII, Arc, c-jun and
c-fos, during learning and operant conditioning task. Experimental
groups were as follows: control (C, the animals never
left the bioterium), when the animals reached 5065% of the
expected response (Incompletely Trained, IT), when animals
reached 100% of the expected response with a latency time
lower than 5 s (Trained, Tr), Box Control of Incompletely
Trained (BCIT), animals spent the same time as the IT in the
operant conditioning box and Box Control of Trained (BCTr)
animals spent the same time as the Tr in the operant conditioning
box. All rats were killed at the same time by cervical
dislocation 15 min after training and hippocampi were removed
and processed. We found increments of mRNA levels
of most genes (BDNF, CREB, Synapsin I, Arc, c-jun and c-fos)
in IT and Tr groups compared to their box controls, but
increments in Tr were smaller compared with IT. These results
describe a differential gene expression in the rat hippocampus
when the animals are learning and when animals
have already learned. Taking together the results presented
herein with the known functions of these genes, we propose
a link between changes in gene expression in the hippocampus
and different degrees of cellular activation and plasticity
during learning of an operant conditioning task. © 2009 IBRO.
Published by Elsevier Ltd. All rights reserved.
Key words: hippocampus, real-time PCR, learning.
2/calmodulin-dependent protein kinase II (CamKII), activity-
regulated cytoskeleton-associated protein (Arc), c-jun and
c-fos have been associated to several learning paradigms in
different brain areas. In this study, we measured mRNA expression
in the hippocampus by real time (RT)-PCR mRNA
levels of BDNF, CREB, Synapsin I, CamKII, Arc, c-jun and
c-fos, during learning and operant conditioning task. Experimental
groups were as follows: control (C, the animals never
left the bioterium), when the animals reached 5065% of the
expected response (Incompletely Trained, IT), when animals
reached 100% of the expected response with a latency time
lower than 5 s (Trained, Tr), Box Control of Incompletely
Trained (BCIT), animals spent the same time as the IT in the
operant conditioning box and Box Control of Trained (BCTr)
animals spent the same time as the Tr in the operant conditioning
box. All rats were killed at the same time by cervical
dislocation 15 min after training and hippocampi were removed
and processed. We found increments of mRNA levels
of most genes (BDNF, CREB, Synapsin I, Arc, c-jun and c-fos)
in IT and Tr groups compared to their box controls, but
increments in Tr were smaller compared with IT. These results
describe a differential gene expression in the rat hippocampus
when the animals are learning and when animals
have already learned. Taking together the results presented
herein with the known functions of these genes, we propose
a link between changes in gene expression in the hippocampus
and different degrees of cellular activation and plasticity
during learning of an operant conditioning task. © 2009 IBRO.
Published by Elsevier Ltd. All rights reserved.
Key words: hippocampus, real-time PCR, learning.