IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Neuropathic pain and temporal expression of preprodynorphin, protein kinase C and N-methyl-d aspartate receptor subunits alter spinal cord injury.
Autor/es:
LABOMBARDA F; CORONEL F; VILLAR M; DE NICOLA A; GONZÁLEZ S
Revista:
NEUROSCIENCE LETTERS
Referencias:
Año: 2008 vol. 447 p. 115 - 119
ISSN:
0304-3940
Resumen:
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Central neuropathic
pain is refractory to conventional treatment and thus remains a therapeutic
challenge.In this work, we used a well-recognized model of central neuropathic
pain to evaluate time-dependent expression of preprodynorphin (ppD), protein
kinase C gamma (PKC_) and NMDA receptor (NMDAR) subunits NR1, NR2A and NR2B,
all critical players in nociceptive processing at the spinal level. Male Sprague-Dawley
rats were subjected to spinal hemisection at T13 level and sham-operated rats
were included as control animals. The development of hindpaw mechanical
allodynia was assessed using the von Frey .laments test. Real time RT-PCRwas
employed to determine the relative mRNA levels of NMDAR subunits, ppD and PKC_ in
the dorsal spinal cord 1, 14 and 28 days after injury. Our results show that, coincident
with the allodynic phase after injury, there was a strong up-regulation of the
mRNAs coding for ppD, PKC_ and NMDAR subunits in the dorsal spinal cord caudal
to the injury site. The present study provides further evidence that these
molecules are involved in the development/maintenance of central neuropathic
pain and thus could be the target of therapeutic approaches.