Systems-level effects of ectopic galectin-7 reconstitution in cervical cancer and its microenvironment

JUAN S. RUIZ-MORENO; RAQUEL SALVADOR-GALLEGO; JANA KLIMENTOVA; CHRISTIANE DINSART; REGINA LY; JUAN CARLOS HIGAREDA-ALMARAZ; FRANK RÖSL; DANIELA BARBIERI; GABRIEL A. RABINOVICH; ILSE A. VALTIERRA-GUTIERREZ; BLADIMIRO RINCON-OROZCO; JIRI STULIK

BMC CANCER

BIOMED CENTRAL LTD

Lugar: Londres; Año: 2016 vol. 16 p. 680 - 689

1471-2407

ckgroundGalectin-7 (Gal-7) is negatively regulated in cervical cancer, and appears to be a link between the apoptotic response triggered by cancer and the anti-tumoral activity of the immune system. Our understanding of how cervical cancer cells and their molecular networks adapt in response to the expression of Gal-7 remains limited.MethodsMeta-analysis of Gal-7 expression was conducted in three cervical cancer cohort studies and TCGA. In silico prediction and bisulfite sequencing were performed to inquire epigenetic alterations. To study the effect of Gal-7 on cervical cancer, we ectopically re-expressed it in the HeLa and SiHa cervical cancer cell lines, and analyzed their transcriptome and SILAC-based proteome. We also examined the tumor and microenvironment host cell transcriptomes after xenotransplantation into immunocompromised mice. Differences between samples were assessed with the Kruskall-Wallis, Dunn?s Multiple Comparison and T tests. Kaplan?Meier and log-rank tests were used to determine overall survival.ResultsGal-7 was constantly downregulated in our meta-analysis (p