GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes

GUERRA LN; SUAREZ C; SOTO A; SCHIAPPACASSE A; SAPOCHNIK D; SACCA P; PIWIEN-PILIPUK G; PERAL B; CALVO JC

CLINICAL & TRANSLATIONAL ONCOLOGY

SPRINGER

Lugar: Berlin; Año: 2015 vol. 17 p. 511 - 520

1699-048X

Introduction: In the mammary gland, the involution that occurs when lactation ends is animportant period for cancer development. Creydt et al (Clin Transl Oncol 2013)demonstrated stromal-epithelium interactions evaluating conditioned medium of adiposetissue on breast epithelial metalloproteases activity. Here, we evaluated the effects ofconditioned medium of breast epithelial mammary cells on stromal cells.Materials and methods: Conditioned medium from normal murine mammary gland cellline (NMuMG), as well as, conditioned medium proteins were obtained. Then, they wereevaluated on modulation of adipocyte differentiation, using 3T3-L1 cell line.   Results: We described, for the first time, that breast epithelial mammary cells couldproduce the enzyme galactose 3-O-sulfotransferase 2 (GAL3ST2). Importantly, GAL3ST2is present in NMMuMG and two human breast cancer cell lines, and it is more stronglyexpressed in more metastatic tumors. When 3T3-L1 preadipocyte differentiation wastriggered in the presence of conditionted medium from NMuMG or GAL3ST2, trigliceryde accumulation was decreased by 40% and C/EBP expression by 80% in adipocytes. Inaddition, the expression of FABP4 (aP2), another marker of adipocyte differentiation, was inhibited by 40% in GAL3ST2-treated cells. Conclusions: Taken together, these results suggest that GAL3ST2 would interfere withnormal differentiation of 3T3-L1 preadipocytes; raising the possibility that it may affectnormal differentiation of stromal preadipocytes and be a link to tumor metastatic capacity.