IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: effect on triglyceride accumulation and cell proliferation.
Autor/es:
JULIANELLI VL; GUERRA LN; CALVO JC
Revista:
BIOCELL
Referencias:
Año: 2007 vol. 31 p. 237 - 245
ISSN:
0327-9545
Resumen:
ABSTRACT: Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis,
and differentiation. Mammary gland has being the chosen model for developmental biologist because the
most striking changes in morphology and function take place after birth. We have demonstrated a regulation
of triglyceride accumulation by protein factors synthesized by normal mouse mammary gland epithelial cells
(NMMG), acting on a cell line, 3T3-L1, long used as a model for adipogenesis. In this paper, we demonstrate
that this inhibitory effect seems to be shared by other cells of epithelial origin but not by other cell types. We
found a regulation of cell proliferation when NMMG cells are cultured in the presence of conditioned media
from Swiss 3T3 or 3T3-L1 cells. We found a possible point of regulation for the mammary factor on a key
enzyme of the lipid metabolic pathway, the glycerol-3-phosphate dehydrogenase. The inhibitory factor seems
to have an effect on this enzymes activity and reduces it. The results presented herein contribute to the
understanding of cell-cell communication in a model of a normal mammary gland.Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis,
and differentiation. Mammary gland has being the chosen model for developmental biologist because the
most striking changes in morphology and function take place after birth. We have demonstrated a regulation
of triglyceride accumulation by protein factors synthesized by normal mouse mammary gland epithelial cells
(NMMG), acting on a cell line, 3T3-L1, long used as a model for adipogenesis. In this paper, we demonstrate
that this inhibitory effect seems to be shared by other cells of epithelial origin but not by other cell types. We
found a regulation of cell proliferation when NMMG cells are cultured in the presence of conditioned media
from Swiss 3T3 or 3T3-L1 cells. We found a possible point of regulation for the mammary factor on a key
enzyme of the lipid metabolic pathway, the glycerol-3-phosphate dehydrogenase. The inhibitory factor seems
to have an effect on this enzymes activity and reduces it. The results presented herein contribute to the
understanding of cell-cell communication in a model of a normal mammary gland.