IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes
Autor/es:
L.N. GUERRA; C. SUAREZ; A. SCHIAPPACASSE; D. SAPOCHNICK; P. SACCA; G. PIWIEN PILIPUK; B. PERAL; J.C. CALVO
Revista:
CLINICAL & TRANSLATIONAL ONCOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2014
ISSN:
1699-048X
Resumen:
Introduction: In the mammary gland, the involution that occurs when lactation ends isan important period for cancer development. Creydt et al (Clin Transl Oncol 2013)demonstrated stromal-epithelium interactions evaluating conditioned medium ofadipose tissue on breast epithelial metalloproteases activity. Here, we evaluated theeffects of conditioned medium of breast epithelial mammary cells on stromal cells.Materials and methods: Conditioned medium from normal murine mammary gland cellline (NMuMG), as well as, conditioned medium proteins were obtained. Then, theywere evaluated on modulation of adipocyte differentiation, using 3T3-L1 cell line.Results: We described, for the first time, that breast epithelial mammary cells couldproduce the enzyme galactose 3-O-sulfotransferase 2 (GAL3ST2). Importantly,GAL3ST2 is present in NMMuMG and two human breast cancer cell lines, and it ismore strongly expressed in more metastatic tumors. When 3T3-L1 preadipocytedifferentiation was triggered in the presence of conditionted medium from NMuMG orGAL3ST2, trigliceryde accumulation was decreased by 40% and C/EBP expression by80% in adipocytes. In addition, the expression of FABP4 (aP2), another marker ofadipocyte differentiation, was inhibited by 40% in GAL3ST2-treated cells.Conclusions: Taken together, these results suggest that GAL3ST2 would interfere withnormal differentiation of 3T3-L1 preadipocytes; raising the possibility that it may affectnormal differentiation of stromal preadipocytes and be a link to tumor metastaticcapacity.