Differential beta2-adrenergic receptor expression defines the phenotype of non-tumorigenic and malignant human breast cell lines

GARGIULO LUCÍA; COPSEL SABRINA; RIVERO EZEQUIEL; GALÉS CÉLINE ; SÉNARD JEAN MICHEL; LUTHY ISABEL; DAVIO CARLOS; BRUZZONE ARIANA

Oncotarget

Impact Journals

Lugar: Albany, N.Y.; Año: 2014 vol. 5 p. 10058 - 10069

1949-2553

Breast cancer is the most frequent malignancy in women. Several reports demonstrated that adrenergic receptors (ARs) are involved in breast cancer. Here we observed that epinephrine (Epi), an endogenous AR agonist, caused opposite effects in non-tumorigenic (MCF-10A and HBL-100) and tumor cells (MCF-7 and MDA-MB-231). Thus, Epi, in non-tumor breast cells, as well as isoproterenol (β-agonist), in all cell lines, maintained a benign phenotype, decreasing cell proliferation and migration, and stimulating cell adhesion. β-AR expression and cAMP levels were higher in MCF-10A than in MCF-7 cells. β2-AR knock-down caused a significant increase of cell proliferation and migration, and a decrease of cell adhesion both in basal and in Iso-stimulated conditions. Coincidently, β2-AR over-expression induced a significant decrease of cell proliferation and migration, and an increase of cell adhesion. Therefore, β2-AR is implied in cell phenotype and its agonists or antagonists could eventually complement cancer therapy.