Involvement of the ANGPTs/Tie-2 system in Ovarian Hyperstimulation Syndrome (OHSS)

SCOTTI L; ABRAMOVICH D; PASCUALI N; DE ZUÑIGA I; OUBIÑA A; KOPCOW L; LANGE S; OWEN G; TESONE M; PARBORELL F

MOLECULAR AND CELLULAR ENDOCRINOLOGY.

ELSEVIER IRELAND LTD

Lugar: Amsterdam; Año: 2013 vol. 365 p. 223 - 230

0303-7207

Ovarian hyperstimulation syndrome (OHSS) is a disorder associated with ovarian stimulation. OHSS featuresare ovarian enlargement with fluid shifting to the third space. Disturbances in the vasculature areconsidered the main changes that lead to OHSS. Our aim was to analyze the levels of angiopoietins 1 and2 (ANGPT1 and 2) and their soluble and membrane receptors (s/mTie-2) in follicular fluid (FF) and ingranulosa-lutein cells culture (GLCs) from women at risk of developing OHSS. We also evaluated theeffect of ANGPT1 on endothelial cell migration. In ovaries from an OHSS rat model, we analyzed the proteinconcentration of ANGPTs, their mTie-2 receptor, and platelet-derived growth factor PDGF-B, -D andPDGFR-b. ANGPT1 levels were increased in both FF and GLCs from women at risk of OHSS. Incubation ofthese FF with an ANGPT1 neutralizing antibody decreased endothelial cell migration. In the ovaries ofOHSS rat model, mTie-2 protein levels increased and PDGF-B and -D decreased. In summary, these resultssuggest that ANGPT1 could be another mediator in the development of OHSS. 2012 Published by Elsevier Ireland Ltd.