IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Heterogeneous distribution of histone methylation in mature human sperm.
Autor/es:
LA SPINA FA; ROMANATO M ; BRUGO-OLMEDO S; DE VICENTIIS S; JULIANELLI V; RIVERA RM; BUFFONE MG
Revista:
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS.
Editorial:
SPRINGER/PLENUM PUBLISHERS
Referencias:
Lugar: New York; Año: 2014 vol. 31 p. 45 - 49
ISSN:
1058-0468
Resumen:
Abstract PURPOSE: To analyze the presence of various histone modifications in ejaculated human spermatozoa METHODS: In this prospective study, seminal ejaculates from 39 normozoospermic individuals were evaluated for semen analysis and the presence of histone modifications in isolated nuclei. RESULTS: We observed heterogeneous presence of histone methylation in normal mature human sperm. We observed that 12 to 30 % of the nuclei of normal sperm contain a heterogeneous distribution of the marks H3K4Me1, H3K9Me2, H3K4Me3, H3K79Me2, and H3K36Me3. Moreover, the presence of these marks is higher in the poor motile fraction of the ejaculate, which is associated with poor morphology and functional quality. In contrast, we did not observe histone acetylation (H3K4Ac and H4K5Ac) in normal or abnormal mature human sperm CONCLUSIONS: Defects in the process of spermatogenesis may alter the correct epigenetic programing in mature sperm. Further studies are required to evaluate the impact of these findings in human infertility.