The use of metaphase I oocytes matured in vitro is associated with embryo multinucleation.

DE VINCENTIIS S; DE MARTINO E; BUFFONE MG; BRUGO-OLMEDO S

FERTILITY AND STERILITY

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2013 vol. 99 p. 414 - 421

0015-0282

OBJECTIVE: To evaluate the impact of oocyte maturational stage at retrieval on embryo multinucleation. DESIGN: Retrospective study. SETTING: Private institution for assisted reproduction. PATIENT(S): A total of 412 patients undergoing 500 intracytoplasmic sperm injection (ICSI) cycles between August 2006 and September 2010. INTERVENTION(S): Routine ICSI laboratory procedures. MAIN OUTCOME MEASURE(S): Normal and abnormal fertilization; embryo development; arrest at pronuclear stage; failure to undergo first mitotic division; presence of embryo multinucleation; embryo quality; pregnancy, implantation, and miscarriage rates. RESULT(S): A significantly lower percentage of multinucleation was found in embryos originating from metaphase II (MII) oocytes when compared with MI-II- and MI-derived oocytes. Significantly fewer multinucleated cells per embryo were observed in MII-derived oocytes. Clinical pregnancy and implantation rates were significantly higher when only embryos derived from MII oocytes were transferred. CONCLUSION(S): Embryo multinucleation rate increases when in vitro-matured (2-5 hours incubation) MI (MI-II) oocytes are used instead of in vivo-matured oocytes in ICSI. Furthermore, all other ICSI outcome parameters are also compromised. The use of donated gametes does not modify these results.