Cytogenetic characterization of the murine bladder cancer model MB49 and the derived invasive line MB49-I

FABRIS VT; LODILLINSKY C; PAMPENA MB; BELGOROSKY D; LANARI C; EJAN AM

CANCER GENETICS AND CYTOGENETICS

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2012 vol. 205 p. 168 - 176

0165-4608

Bladder cancer is frequently associatedwithchromosomalabnormalities,andthecomplexity of karyotypes increases with tumor progression. The murine model MB49 is one of the most widely studied models of bladder cancer.Wedeveloped the invasive cell lineMB49-I by successive in vivo passages ofMB49 primary tumors. Because little is known about the chromosomal alterations of thismodel, our goal was to performcytogenetic analyses of theMB49 andMB49-I lines. The karyotypes of both lines were analyzed by G-banding and fluorescence in situ hybridization techniques. Both lines were composed of two cell subpopulations, a diploid population, which was found mainly in the MB49 line, and the tetraploid population, which was found mainly in the MB49-I line. A translocation between chromosomes 5 and 9 and an isochromosome of chromosome 19 were observed in the subpopulations of both lines. New structural abnormalities and additional chromosomal imbalances were detected in the MB49-I line. Tumor progression in the MB49/MB49-I model was associated with a selection of polyploid cells with accompanying chromosomal abnormalities. This model may be advantageous for the study of the genetic changes associatedwith the progression of bladder cancer.