Identification of molecular markers in bladder cancer using murine and human models

LAPYCKYJ L.; GIUSTINA S.; LODILLINSKY C.; ROSSO M.; TEJERIZO J. C.; GONZALEZ M. I.; EIJAN A. M.; VAZQUEZ-LEVIN M. H.

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Buenos Aires; Año: 2011 vol. 35 p. 79 - 79

0327-9545

Introduction: Bladder cancer affects over 1 million people worldwide, but the molecular basis remains unknown. Downregulation or loss of epithelial cadherin (cadE) is a prognostic marker for this cancer. Presence of negative modulators of its expression (Snail) and of mesenchymal cadherins (Neural cadherin, cadN) has been associated to tumor progression. Dysadherin (Dys) is a transmembrane glycoprotein that regulates cadE adhesive functions, but its expression in bladder cancer has yet not been reported. Objective: To evaluate the expression of cadE and its modulators in 3 bladder cancer models: a) an orthotopic animal model (MB49/MB49-I) b) a human invasive cell line (T24) c) human tumors. Methods: RT-PCR, Western Immunoblotting and immunocytochemistry. Results: In the 3 models, low levels of cadE expression were found, and cadN and Snail trancripts were detected. Expression of Dys was determined in bladder tumors of human and murine origin. Discussion: The study describes changes in expression of cadE, cadN and Snail and reports, for the first time, the detection of Dysadherin, a new molecular marker in the evaluation of bladder cancer.