IBYME   02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Boar sperm functionality is related to α-tocopherol content after freezing-thawing.
Autor/es:
BREININGER ELIZABETH; DESCALZO A.; ROSETTI L.; ABRAMOVICH DALHIA; BECONI MARTA T.
Revista:
ANDROLOGIA
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2011 vol. 43 p. 409 - 415
ISSN:
0303-4569
Resumen:
Boar spermatozoa are sensitive to oxidative damage produced during
cryopreservation. Our aim was to evaluate the participation of different
antioxidants in the improvement of cryopreserved boar sperm
functionality. Spermatozoa frozen with 200 μg ml(-1) α-tocopherol, 0.5
mm 17β-oestradiol or seminal plasma were used to evaluate sperm
parameters and capacitation-like changes. The 17β-oestradiol and
α-tocopherol concentrations were assessed by RIA and HPLC respectively.
Motility was improved but lipid peroxidation and capacitation-like
changes were diminished (P < 0.05) in antioxidant samples. A
significant increase in 17β-oestradiol concentration was detected in
17β-oestradiol or seminal plasma samples. Alpha-tocopherol content
increased in α-tocopherol, 17β-oestradiol or seminal plasma samples,
obtaining the lowest level in the α-tocopherol ones. The 17β-oestradiol
or seminal plasma components may be acting in the regeneration of the
α-tocopherol antioxidant capacity. The α-tocopherol concentration may be
conditioning the cryopreserved boar sperm functionality. The addition
of antioxidants could be useful to reduce oxidative stress, thus
improving the functionality of cryopreserved boar spermatozoa.