High level expression of bioactive recombinant human growth hormone in the milk of a cloned transgenic cow

DANIEL SALAMONE; LINO BARAÑAO; CLAUDIO SANTOS; LEONARDO BUSSMANN; JORGE ARTUSO; CARLOS WERNING; AIDA PRYNC; CESAR CARBONETTO; SUSANA DABSYS; CARLOS MUNAR; ROBERTO SALABERRY; GUILLERMO BERRA; IGNACIO BERRA; NAHUEL FERNÁNDEZ; MARIANA PAPOUCHADO; MARCELO FOTI; NORBERTO JUDEWICZ; IGNACIO MUJICA; LUCIANA MUÑOZ; SILVINA FENÁNDEZ ALVAREZ; ELISEO GONZÁLEZ; JUAN ZIMMERMANN; MARCELO CRISCUOLO; CARLOS MELO

JOURNAL OF BIOTECHNOLOGY

Año: 2006 vol. 124 p. 469 - 472

0168-1656

Transgenic farm animals have been proposed as an alternative to current bioreactors for large scale production of biopharmaceuticals.However, the efficiency of both methods in the production of the same protein has not yet been established. Herewe report the production of recombinant human growth hormone (hGH) in the milk of a cloned transgenic cow at levels ofup to 5 g l−1. The hormone is identical to that currently produced by expression in E. coli. In addition, the hematological andsomatometric parameters of the cloned transgenic cow are within the normal range for the breed and it is fertile and capable ofproducing normal offspring. These results demonstrate that transgenic cattle can be used as a cost-effective alternative for theproduction of this hormone.