Modulation of Inflammatory Mediators of Maternal Immune Activation by Resveratrol

CORREA FERNANDO; TICCHI JULIA

Mar del Plata

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021 SAIC - SAI - AAFE - NANOMED-ar; 2021

Sociedad Argentina de Investigación Clínica

Maternal immune activation (MIA) produces structural, metabolic and epigenetic changes in the fetus associated with increased risk for various neurodevelopmental and neurodegenerative disorders. The hallmark of brainneuroinflammation is the activation of microglia, one of the main effectors of the innate immune response in the CNS. Excessive microglial activation leads to CNS damage due to excessive production of pro-inflammatory mediators, like interleukins, MMP, PGE2, NO and ROS.It has been demonstrated that resveratrol has anti-inflammatory, antioxidant and epigenetics properties that translate into neuroprotective effects in adults.Objectives: To study the possible neuroprotective effects of resveratrol in the fetal CNS in a model of MIA induced by bacterial lipopolysaccharide (LPS) and to determine the pro-inflammatory mediators involved in the modulation of the response.Resveratrol was administered to Balb/c females on gestational day 15, which were then exposed or not to LPS. After LPS administration, amniotic liquid was collected to evaluate activity of MMP2 and MMP9. Additionally the brain of the offspring were collected to evaluate the expression of two inducible enzymatic pathways (COX-2 and iNOS) that produce mediators (prostaglandins and nitric oxide) known to cause inflammation and to evaluate the acetylation of H3 and H4 since DNA acetylation is associated with the regulation of inflammatory gene expression.Results: Preliminary results show that the LPS-triggered MIA induces COX2 expression in the fetal brains (p