Extruded cAMP through MRP4 modulates motility and actin polymerization in bull cryopreserved spermatozoa

NICOLAS CHIARANTE, CARLOS AGUSTÍN ISIDRO ALONSO, RAQUEL LOTTERO, CAMILA ARROYO SALVO, EUGENIA BOGETTI, JESSICA PLAZA, CARLOS DAVIO, MARCELO MIRAGAYA,SILVINA PEREZ MARTINEZ

MODO VIRTUAL

Congreso; Society for the Study of Reproduction (SSR) 53rd Annual Meeting; 2020

Abstract # 2354Capacitation is a key process involved in the acquisition of sperm fertilizingcompetence. The importance of cAMP during capacitation has led us to study the roleof this nucleotide as an extracellular stimulus, as well as the multidrug resistance-associated protein 4 transporter (MRP4) that extrudes cAMP from cells. Our grouppreviously described that MRP4 mediates cAMP efflux in bovine spermatozoa and thatextracellular cAMP triggers events associated to capacitation. In this work, we deepenthe study of the role of MRP4 in the acquisition of sperm fertilizing ability using MK571, anMRP4 inhibitor. We evaluated capacitation by LPC-induced acrosome reaction andthe ability of sperm to be released from oviductal epithelia. Both events weredecreased when the MRP4 inhibitor (50 µM) was added (p