CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CELL PROLIFERATION AND DEATH IN PLACENTA ASSOCIATED WITH FETAL GROWTH RESTRICTION
Autor/es:
ANACLARA MARINO; MANUEL L. WOLFSON; JULIETA A. SCHANDER; JULIETA AISEMBERG; CAROLINA MARVALDI; ANA M. FRANCHI
Lugar:
Modo virtual
Reunión:
Congreso; SSR Virtual 2020; 2020
Institución organizadora:
Society for the Study of Reproduction
Resumen:
Intrauterine growth restriction (IUGR) is a condition whereby a fetus is unable to achieveits genetically determined potential size. Prenatal stress is one of the causes that alterthe intrauterine environment, which affects the normal development and function ofthe placenta, and the fetal growth. Furthermore, IUGR is associated with placentaldysfunction, where altered trophoblast cells turnover and function contribute toreduced feto-placental growth. The balance between placental cell proliferation anddeath is a key point during the development of the fetus.The aim of this work was to study the differences in cell proliferation, apoptosis andautophagy in placental tissue in normal and IUGR placentas. The IUGR mouse model usedwas animals treated with a synthetic glucocorticoid during late pregnancy to cause growthrestriction.Pregnant BALB/c mice received 8 mg/kg (s.c.) of dexamethasone between days 14 and15 of pregnancy. The control group was sham-treated with saline. Prenatalglucocorticoid treatment not only induced fetal growth restriction but also decreasedplacental weight. Placental tissue from pregnant animals was dissected on gestationaldays 15 to 18 and processed for Western Blot and RT-qPCR analysis. The results wereanalyzed with a one-way ANOVA and Tukey test (p