CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Changes in sperm quality of the offspring caused by diet-induced maternal overweight
Autor/es:
CORTEZ ANALIA ELIZABETH; MENEGHINI MARIA AGUSTINA; FALETTI ALICIA GRACIELA; FLORES QUIROGA JEREMIAS PABLO
Lugar:
En realidad fue on line por la pandemia
Reunión:
Congreso; Latinoamerican DOHAD; 2020
Institución organizadora:
SOCIEDAD BRASILEIRA DE FISIOLOGIA
Resumen:
Changes in Sperm Quality of the Offspring Caused by Diet-Induced Maternal OverweightMeneghini, María Agustina, Flores Quiroga, Jeremías Pablo, Cortez, Analía Elisabeth, Faletti, Alicia GracielaCentro de Estudios Farmacológicos y Botánicos (CEFYBO-CONICET), Facultad de Medicina, Universidad de Buenos Aires.Maternal obesity has become a stressor factor during the fetal programming that represents an important health determinant in the offspring during childhood and adult life. The risk of developing pathologies in the offspring has a direct relation to the degree of overweight or severity of maternal obesity.Previously, we demonstrated that male offspring from rats fed with a high-fat diet exhibited higher body and testis weight, greater ano-genital distance, altered puberty and a high level of testosterone with normal glycemia. Also, offspring from rats with different degree of obesity showed a lower number of germ and somatic cells, altered morphology in spermatozoa and motility, and abnormal spermatogenic process. Thus, the aim of the present study was to continue studying the effect of maternal overweight, induced by diet with high-fat content, on both the quality and function of sperm. To this end, at 22 days of age, female Sprague Dawley rats were fed with a standard (SD) or high-fat diet (cafeteria diet, CD) continuously until weaning of their offspring. All male offspring (OSD and OCD, respectively) were fed with SD, inspected periodically and euthanized at 60 days of age.Sperm capacitation by acrosomal reaction, the presence of the reactive oxygen species by flow cytometry in the germ cells using a fluorescent probe (2?,7?-dichlorofluorescein diacetate), DNA fragmentation by Tunel kit, mitochondrial function using the probe 3.3?-diaminobenzidine, the membrane functional status by hypoosmotic swelling test, and the presence of abnormal chromosomes by cytogenetic assay (Evan test), were examined. Compared with OSD rats and expressed as percentage, OCD group showed a decrease in the acrosome-reacted sperm (57±3 vs 69±2, p