Disruption in the sperm quality of the offspring caused by maternal overnutrition in rats

JEREMÍAS PABLO FLORES QUIROGA; ANALÍA ELIZABETH CORTEZ; MARÍA AGUSTINA MENEGHINI; ALICIA GRACIELA FALETTI

CABA

Congreso; Reunión de Sociedades de Biociencias 2020; 2020

Sociendad Argentina de Investigaciones Clínicas

DISRUPTION IN THE SPERM QUALITY OF THE OFFSPRING CAUSED BY MATERNAL OVERNUTRITION INRATSMeneghini MA, Flores Quiroga JP, Cortez AE, Faletti AGCentro de Estudios Farmacológicos y Botánicos (CEFYBO-CONICET), Facultad de Medicina, Universidad de Buenos Aires.Obesity has increased in recent years and is the most important noncommunicable chronic disease. Maternal overnutrition may induce multiple pathologies in both women and their offspring. Our previous studies showed that male offspring from high-fat-fed rats exhibited higher body and testis weight and altered puberty. Also, we found a lower number of germ cells, percentage of motile sperm and capacitation. Thus, the aim of the present study was to evaluate the effects of maternal overnutrition, induced by high-fat diet, on the quality and function of sperm in the offspring.To this end, maternal overnutrition were induced by a high-fat palatable (cafeteria) diet, which was supplied continuously until weaning of their offspring, including pregnancy and lactation. Male offspring from rats fed standard (OSD) or cafeteria diet (OCD) were fed with a standard diet, inspected periodically, and euthanized at 60 days of age. In the germ cells we examined the presence of the reactive oxygen species by flow cytometry using a fluorescent probe (2,7-dichlorofluorescein diacetate), DNA fragmentation by TUNEL kit, mitochondrial function using the probe 3.3-diaminobenzidine, the membrane functional status by hypoosmotic swelling test, and the presence of abnormal chromosomes by cytogenetic assay (Evan test). Compared with OSD rats, OCD group showed a lower percentage of the hypoosmotic-reacted sperm (15±1 vs 23±2, p