MATERNAL DIETS ENRICHED IN SUNFLOWER AND CHIA OIL MODULATE PPAR PATHWAYS IN THE DECIDUA FROM DIABETIC RATS DURING EARLY POSTIMPLANTATION

HUGO SATO; ALICIA JAWERBAUM; CINTIA ROMINA GATTI; SABRINA LORENA ROBERTI; ROMINA HIGA

BUENOS AIRES

Congreso; International Federation of Placenta Associations; 2019

International Federation of Placenta Associations

Objectives: PPARs are nutritional sensors whose activation regulates genes involved in lipid metabolism and transfer. Previously, in the decidua from 9-day-pregnant diabetic rats we found that PPARgamma and its target genes PLIN2 and FABP4 were increased while PPARalpha and its target genes ACO and CPT-1 were reduced. Here, we hypothesized that maternal diets enriched with sunflower and chia oil (Sources of n-6 and n-3 PUFAs, PPARs natural ligands) modulate PPARs levels and their target genes in the decidua from 9-day-pregnant diabetic rats. Methods: Diabetes was induced in female rats by streptozotocin administration (50 mg/kg) two weeks before mating. On days 7 to 9 of pregnancy, diabetic rats received a standard diet or a diet enriched either in 6% of sunflower oil or 6% chia oil. On day 9 of pregnancy the decidua was explanted, PPARs were measured by Western blot, target genes mRNA levels by RT-qPCR and proteins expression by immunohistochemistry. Results: Maternal diets enriched in sunflower and chia oils prevented the increased PPARgamma levels in the decidua from diabetic rats. Although PLIN2 levels did not change with the maternal treatments, FABP4 levels were increased in the decidua from diabetic rats fed the sunflower and chia oil-enriched diets (85% and 123%; p