CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Stx2 from enterohemorrhagic Escherichia coli produces cerebellar impairment of the vascular unit with inflammatory involvement
Autor/es:
CORREA, FERNANDO; GOLDSTEIN, JORGE; VELARDO, VANINA GISELLE; CANGELOSI, ADRIANA; BERDASCO, CLARA VALENTINA; GEOGHEGAN, PATRICIA
Lugar:
Villa Carlos Paz
Reunión:
Congreso; XXXIV Reunión Anual Sociedad Argentina Investigación en Neurociencias 2019; 2019
Resumen:
Hemolytic uremic syndrome (HUS) is a triad of events that includes thrombocytopenia, microangiopathic hemolytic anemia and acute renal failure caused by Shiga toxin-producing Escherichia coli (STEC). Also, HUS produces neurologic alterations. It has been observed that Shiga toxin 2 (Stx2) produces deficits of coordination and equilibrium in patients which suggest cerebellar impairment. Furthermore, we have characterized a model of injury in mice that mimicked clinical impairment of the cerebellum (Front. Microbiol.7:133.doi:10.3389/fmicb.2016.00133). The aims of this study were to determine whether Stx2causes cell damage in an inflammatory frame in the cerebellum. To determine this, male Swiss NIH mice (n=4) were injected intravenously with 1ηg of Stx2 per mice or 100 µl of saline solution (Control group). After 2 and 4 days of treatment, fixed brains were subjected to immunostaining with lectins to determine the microvasculature profile, immunofluorescence with anti-GFAP and anti-MBP to determine reactive astrocytes and myelin oligodendrocytes conservation state respectively. Images were captured from a confocal microscope and analyzed through the software ImageJ (NIH). To determinate the inflammatory profile, an ELISA test was employed to measure TNFα and IL-10.Stx2 was able to reach the Purkinje and granular cerebellar layers and damaged the endothelial cells after 4 days. The statistical results analyzed by Student´s t-test showed thatStx2 significantly: i. decreased the area occupied by the microvasculature (12.58 ± 0.73 Control vs 7.71 ± 0.72 Stx2, day 2, and 12.9 ± 0.5 Control vs 10.03 ± 0.3 Stx2 day 4, in µm2, p