INVOLVEMENT OF INFLAMMATION AND OXIDATIVE STRESS IN THE ADRENOCORTCIAL DYSFUNCTION IN INSULIN RESISTANT RATS: EFFECT OF HEMIN TREATMENT

MORENA WISZNIEWSKI; CAROLINA VECINO; ESTEBAN MARTIN REPETTO; CALDARERI, L.; CORA CYMERYNG

Nueva Orleans

Congreso; ENDO 2019 Abstracts - 101st Annual Meeting of the Endocrine Society; 2019

Endocrine Society

An increased consumption of sweetened beverages has been linked to changes in the prevalence of obesity and insulin resistance (IR) worldwide. In rats with IR, induced by administration of a sucrose rich diet, we have previously demonstrated a dysregulation of adrenocortical steroid production. As oxidative stress generation and a chronic inflammatory state have been singled out as hallmarks of this disease, in this study we sought to analyze the effects of HO-1 (an antioxidant enzyme) induction on functional parameters in the adrenal cortex. Rats were randomly distributed into Control (C) and SRD- treated groups. After 12 weeks of treatment, animals from both groups were treated with hemin (15 mg/kg i.p every 48 h; H and SRDH groups). An insulin tolerance test (ITT) was performed in week 12 and the rate constant KITT was calculated as 0,693*t1/2. All animals were sacrificed 2 weeks after and blood and adrenal glands were excised. Statistical significance of data was analyzed by ANOVA followed by Tukey?s test. Results showed that fasting glucose levels were increased in SRD (p