EXPERIMENTAL GESTATIONAL DIABETES INDUCES A PROINFLAMMATORY AND PRO-OXIDANT ENVIRONMENT IN MATERNAL AND FETAL LIVERS

HEINECKE, MARÍA FLORENCIA; CAPOBIANCO, EVANGELINA; FORNES, DAIANA; JAWERBAUM, ALICIA

CABA

Congreso; International Federation of Placenta Associations 2019 (IFPA 2019); 2019

Objectives: Gestational diabetes (GDM) is a prevalent disease associatedwith a pro-oxidant and pro-inflammatory environment and with adverseconsequences to both the mother and the offspring. The fetal liver is highlyexposed to metabolic abnormalities, potentially leading to alterations in itsdevelopment, which would affect the metabolic function in adulthood.The aim of this work was to evaluate whether pro-inflammatory and prooxidantmarkers are increased in maternal and fetal livers in GDM rats.Methods: GDM was spontaneously induced by intrauterine programmingin the offspring (F1) of diabetic rats (F0 diabetic rats were obtainedthrough neonatal streptozotocin administration). In control and GDM rats,maternal and fetal livers were explanted on day 21 of gestation for furtherevaluation of pro-oxidant and pro-inflammatory markers (nitric oxideproduction (nitrates/nitrites measurement), lipoperoxidation (TBARSmeasurement), and the protein expression of metalloproteinase-2(MMP2), connective tissue growth factor (CTGF), and Manganese SuperoxideDismutase (Mn-SOD)) as well as the levels of PGC-1alpha, a PPAR coactivatorthat regulates pro-oxidant/pro-inflammatory processes.Results: In the maternal liver of rats with GDM the levels of nitrates/nitriteswere increased (254%, p