Enrichment of maternal environment protects the offspring through changes in the amniotic fluid.

CORREA, FERNANDO; DE LA CRUZ BORTHIRY, FERNANDA; FRANCHI, ANA MARÍA; AISEMBERG, JULIETA; WOLFSON, MANUEL; SCHANDER, JULIETA; MARVALDI, CAROLINA; JENSEN, FEDERICO

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2019

Maternal lifestyle affects both pregnancy outcomes and maternal health. We previously demonstrated that the exposition to an enriched environment (EE), a non-invasive stimulus of the sensory pathway combined with voluntary physical activity, prevented from preterm birth induced by the administration of bacterial lipopolysaccharide (LPS) in a mouse model. Furthermore, mothers exposed to EE presented less perinatal death when compared to control environment (CE, standard cages) and EE also reverted some of the deleterious effects of the LPS during development. The amniotic fluid (AF)exerts several functions during pregnancy. It protects the fetuses by not only cushioning it from outside pressures but also having immunological functions.The aim was to analyze physiological changes in the AF, associated with the protective effects of the EE on the offspring exposed to LPS. Animals were housed in EE (or CE) cages for 6 weeks and then mated with CE males. On day 15 of pregnancy, LPS was administered and 8h later, amniotic fluid was collected to evaluate several cytokines expression and cellular profile by flow cytometry. We found higher levels of IL-10, an anti-inflammatory cytokine, in AF from EE exposed females when compared to controls (p