Oxytocin as anti-inflammatory agent for periodontal disease.

DMYTRENKO, GANNA; FERNANDEZ-SOLARI, JAVIER; MIRAMÓN, BERNARDO; SURKIN, PABLO NICOLÁS; DE LAURENTIIS, ANDREA

Mar del Plata

Congreso; Reunión Anual de Biociencia 2019 (SAIC, SAFE, SAB, SAP); 2019

SAIC, SAFE, SAB, SAP

Periodontitis (P) is an oral multifactorial infectious disease triggered by bacteria which affects the teeth-support tissue. The penetration of bacterial products causes an immuno-inflammatory response leading to destruction of alveolar bone. We previously demonstrated an anti-inflammatory role of Oxytocin (Oxy) that reduces systemic levels and hypothalamic expression of pro-inflammatory cytokines. Furthermore, atosiban, an antagonist of Oxy receptor, induces an increase in these cytokines per se and blocked Oxy anti-inflammatory effect. Endocannabinoids (ECs) are endogenous lipid signaling molecules that can modulate the immune system and are present in the gingival tissue. ECs would play a role in the regulation of gingival inflammation and bone metabolism.Given that Oxy could act as an anti-inflammatory agent, in this work we study its effect on gingival inflammatory parameters in a rat model of experimental P, as well as the participation of cannabinoid receptors.The P was induced by bilateral linen ligature around the first lower molar during one week. After that, the gingival tissue was removed and jaws were cleaned and stained with methylene blue at 0,1% to visualize the amelocementary limit.We demonstrate for the first time the mRNA expression (PCR) for the Oxy receptor in rats gum, also P significantly increased its expression. Oxy (1000 ug/kg s.c.) significantly reduces bone loss in P (p