PATERNAL DIABETES INDUCED BY INTRAUTERINE PROGRAMMING AFFECTS LIPID METABOLISM IN THE PLACENTA

FORNES, DAIANA; SATO, HUGO; CAPOBIANCO, EVANGELINA; HEINECKE, MARÍA FLORENCIA; JAWERBAUM, ALICIA

Mar del Plata

Congreso; Reunión anual de Sociedades Biocientíficas (SAIC 2019); 2019

Maternal diabetes programs diabetes in the adult offspring with alterations in peroxisome proliferator activated receptor (PPAR) pathways that regulate lipid metabolism. However, little is known about the paternal contribution to long-term offspring?s metabolic health. The aim was to evaluate the regulation of lipid metabolism in the placenta of male fetuses from healthy pregnant rats that were mated with male diabetic rats.Methods: Control (C) and type 2 diabetic male rats (D, diabetes obtained by intrauterine programming, glycemia 140-190 mg/dL) were mated with control female rats. On day 21 of gestation, the pregnant and male rats were euthanized. The placenta, the fetuses and fetal plasma were obtained for evaluation. In paternal and fetal plasma the levels of glycemia, triglyceridemia and cholesterolemia were evaluated by commercial kits. Lipid levels (by TLC) and mRNA levels of genes involved in lipid metabolism (by qPCR), were measured in the placenta of male fetuses from C and D rats.Results: In the paternal plasma of D rats the levels of triglycerides and cholesterol were increased (39% and 21%, respectively p