THE ROLE OF CERVICAL ENDOCANNABINOID SYSTEM IN THE MODULATION OFLIPOPOLYSACCHARIDE-INDUCED PROINFLAMMATORY RESPONSE

HERRERO LO GUIDICE, FELISA MARIA; FRANCHI, ANA MARIA; SCHANDER, JULIETA AYLEN; WOLFSON, MANUEL LUIS; MARVALDI, CAROLINA; AISEMBERG, JULIETA

CABA

Congreso; IFPA 2019- VIII SLIMP; 2019

ObjectivesPreviously, we demonstrated the presence of the endocannabinoid system (ECs) inmurine cervix. Based on this, the objective of the present study was to investigate the mechanisms involved in the proinflammatory response in 15 day-pregnant mice cervix to lipopolysaccharide (LPS) and its possible modulation through the ECs.MethodsThe cervix is a critical organ for pregnancy maintenance . Dynamic alterations in the structure of the cervical extracellular matrix drive flexibility and mechanical strength of the cervix throughout pregnancy, labor, and postpartum. Metalloproteases (MMPs) andcyclooxygenase 2 (COX-2) are involved on events associated with labor induction like cervix ripening.We performed an ex vivo model to evaluate MMPs activity and COX-2 protein levels. Briefly, cervix from 15 day pregnant mice were obtained and cultured in presence or absence of LPS (1μg/ml) for 6 hours. Besides, we used specific cannabinoid receptors (CBs) antagonist AM251 (for CB1, 10-8M) and AM630 (for CB2, 10-8M). MMPs gelatinase activity was evaluated by zymography and COX-2 protein levels by Western Blot.Results We found that LPS increased significantly MMP9 and MMP2 gelatinase activity in cervix (P