The treatment of MCF-7 cells with carbachol and paclitaxel is effective to reduce tumor cell growth in vitro and angiogenesis in vivo.

MARTÍNEZ PULIDO, P.; ALEJANDRO ESPAÑOL; SANCHEZ Y; SALES M; SALEM , A; SANCHEZ F

Mar del Plata

Congreso; S A I C . S A F E . S A B . SAP 2 0 1 9; 2019

SAIC-SAFE-SAB-SAP

Previously we demonstrated that muscarinic receptors (MR) are expressed in different types of human and murine breast tumors. Their activation with the synthetic agonist carbachol (Carb) promotes cell death and improves the effect of paclitaxel (PX) a cytotoxic drug commonly used in breast cancer treatment. In this work, we analyzed the ability of a combination of low concentrations of Carb+PX, simulating a metronomic schedule, to reduce cell growth in bi (2D) and tridimensional (3D) MCF-7 cell cultures (by MTT assay and by microscopy respectively). We also studied the effect of this combination in HMEC-1 cells´ tubulogenesis and the in vivo effect on the neovascular response (Nº vessels/mm2) in mice tumor bearers. We observed that the treatment of MCF-7 cell spheroids with Carb (10-11M)+PX (10-9M) significantly reduced their 3D growth compared to control spheroids by 33±3% at day 6 of culture (p