IMPACT OF MATERNAL OVERNUTRITION ON THE SPERM QUALITY OF MALE OFFSPRING IN RATS

GALARZA ROCIO ALEJANDRA; MENEGHINI MARIA AGUSTINA; FALETTI ALICIA GRACIELA

Congreso; Reunión Anual de Sociedades de Biociencia; 2019

Sociendad Argentina de Investigaciones Clínicas

Abstract/Resumen: Maternal overnutrition may induce multiplepathologies in both mother and offspring. The risk of thesediseases has a direct relation to the degree of overweight orseverity of maternal obesity. The aim of the present work was tostudy the effect of maternal overnutrition, particularly with highfat content, on the sperm quality of the male offspring. To thisend, male offspring from rats fed standard (SD) or cafeteria (CD)diet were used. Considering the overweight of the CD rats whenthey became pregnant (day 0) and to relate the effects observedin the offspring to the different degree of maternal overweight,offspring from CD (OCD) rats were divided into two groups:offspring from rats with 25 % and 35 % overweight (OCD25 andOCD35, respectively). Offspring were euthanized at 60 days ofage. Weight gain, preputial separation, sperm count, spermmotility, sperm capacitation by acrosomal reaction, and thepresence of the reactive oxygen species (ROS) by flow cytometryin the germ cells, using a fluorescent probe (2?,7?-dichlorofluorescein diacetate), were examined. Compared withOSD and expressed as percentage, both OCD groups showed anincrease in the weight gain (13-33, p