OVERNUTRITION INCREASES THE ADVERSE EFFECTS INDUCED BY REPETITIVE EXPOSURE TO 3-METHYLCHOLANTHRENE ON SPERM QUALITY

MARIA AGUSTINA MENEGHINI; JEREMIAS PABLO FLORES QUIROGA; ALICIA GRACIELA FALETTI

Mar del Plata, Buenos Aires

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA 2019; 2019

SAIC, SAFE, SAP, SAB, NANOMED-AR, AACYTAL

3-Methylcholanthrene (3MC), apolyaromatic hydrocarbon, is an environmental pollutant thatcauses reproductive toxicity and is considered an obesogen byaltering the lipid homeostasis, increasing the adipogenesis andcausing metabolic disbalances. Previously, we found that 3MCimpair the ovarian function by affecting the oocyte integrity andfollicular development. Now, the aim of the present work was tostudy the effect of repetitive exposure to low doses of 3MC onthe sperm quality in rats and to assess its relationship withovernutrition. To this end, male prepubertal rats fed standard(SD) or cafeteria (CD) diet were exposed to vehicle or 3MC (0.1mg/kg) three times a week for 40 days. Rats were euthanized at61 days of age and spermatozoa were collected by dissecting thecaudal region of the epididymis. Sperm count (millions ofspermatozoa/ml), motility (percentage), morphology by stainingwith Giemsa, and the presence of abnormal chromosomes bycytogenetic assay, were examined. CD rats treated with 3MC(CD3M) exhibited lower body weight (p