CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Participation of the hypothalamic TRPV1 receptor in the reproductive axis.
Autor/es:
ANA MARIA GENARO; JAVIER FERNANDEZ-SOLARI; JUAN CARLOS ELVERDIN; PABLO NICOLÁS SURKIN; ANDREA DE LAURENTIIS
Lugar:
Cancún
Reunión:
Congreso; XII Congress of the Latin American Association of Immunology (ALAI), XXIII Congress of the Mexican Society of Immunology (SMI); 2018
Institución organizadora:
Latin American Association of Immunology
Resumen:
Participation of the hypothalamic TRPV1 receptor in the reproductive axisPablo N. Surkin, Ana M. Genaro, Juan C. Elverdin, Andrea De Laurentiis andJavier Fernandez-SolariIt is known that immune challenge inhibits the hypothalamic pituitary gonadal (HPG) axis activity, and we have recently reported that the hypothalamic cannabinoid receptor type 1 (CB1) partially mediates this effect in male rats, favoring the control of inflammatory response in detriment of sexual hormone release. However, no studies evaluating the participation of TRPV1vanilloid receptor, which also acts as a nonspecific receptor for the endocannabinoid anandamide, were reported. Therefore, we assessed the participation of the hypothalamic TRPV1 on the HPG axis activity in rats submitted or not to immune challenge induced by LPS.Male adult rats (n=6/group) were treated via intracerebroventricular (icv) with the TRPV1 antagonist Capsazepine (500ng/5ul), followed by an intraperitoneal (ip) administration of lipopolysaccharide (LPS, 5mg/kg) or saline 15 min later. 180 min post ip injections, hypothalamic pro-inflammatory cytokines and neuropeptides involved in reproductive function were assessed by qPCR. Hypothalamic Tnfα and Il1β mRNA expression was increased with bothTRPV1 blockade and by LPS administration, separately (p