SLPI IMPROVES THE RECELLULARIZATION OF RENAL SCAFFOLDS

GUERRIERI DIEGO; SÁNCHEZ FRANCISCO; CHULUYAN EDUARDO; HAEUBLEIN GERALDINE; CARO FIORELLA; CASADEI DOMINGO; AMBROSI NELLA; INCARDONA CLAUDIO

Mar del Plata

Congreso; Reunión Conjunta SAIC-SAI-SAFIS 2018; 2018

Sociedad Argentina de Inmunología (SAI)

(668) SLPI IMPROVES THE RECELLULARIZATION OF RENAL SCAFFOLDSGeraldine Haeublein1, DIEGO GUERRIERI1, Nella Ambrosi1, Fiorella Caro1, Francisco Sanchez1, Claudio Incardona, Domingo Casadei, Eduardo Chuluyan11CEFYBO-CONICET-UBATo deal with the problematic of the lack of donors for organ transplantation, regenerative medicine proposes the generation of histocompatible bioartificial organs by recellularizing scaffolds. In previous studies, we observed that secretory leukocyte protease inhibitor (SLPI) exhibits nephroprotective activity in vivo. The aim of this work was to evaluate the use of SLPI to improve the recellularization of renal scaffolds with human proximal tubule epithelial(HK-2). First, we evaluated in vitro a protective effect of SLPI on HK-2 cell exposed to FK-506 by the MTT assay and annexin V/propidium iodide. Furthermore, we study the ability of SLPI to induce HK-2 proliferation and the effect of SLPI in the wound healing assay. Finally, we produce rat kidney scaffold with the constant admistration in renal artery of 1% SDS (2ml/min) for 24 h followed by 24 h of 0.1% Triton X-100.After washing with saline solution, scaffolds were seeded through the ureter with 2.106 HK-2 cells previously treated or not with SLPI (4μg/ml). Our results showed that the incubation of HK-2 with SLPI (0,4 μg /ml) for 24 h reduce the cytotoxicity induced by FK-506 (p