Hypothalamic TRPV1 participates in the control of the reproductive axis in male rats.

GANNA DMYTRENKO; ANDREA DE LAURENTIIS; ANA MARIA GENARO; SURKIN, PABLO NICOLÁS; FERNÁNDEZ-SOLARI JAVIER; KAUFFMAN AS

Mar del Plata

Congreso; LVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2018

SAIC

It is known that immune challenge inhibits the hypothalamic pituitary gonadal (HPG) axis activity, and we have recently reported that the hypothalamic cannabinoid receptor type 1 (CB1) partially mediates this effect in male rats, favoring the control of inflammatory response in detriment of sexual hormone release. However, no studies evaluating the participation of TRPV1 vanilloid receptor, which also acts as a nonspecific receptor for the endocannabinoid anandamide, were reported. Therefore, we assessed the participation of the hypothalamic TRPV1 on the HPG axis activity in rats submitted or not to immune challenge induced by LPS. Male adult rats (n=6/group) were treated via intracerebroventricular (icv) with the TRPV1 antagonist Capsazepine (500ng/5ul), followed by an intraperitoneal (ip) administration of lipopolysaccharide (LPS, 5mg/kg) or saline 15 min later. 180 min post ip injections, hypothalamic pro-inflammatory cytokines and neuropeptides involved in reproductive function were assessed by qPCR. Hypothalamic Tnfα and Il1β mRNA expression was increased with both TRPV1 blockade and by LPS administration, separately (p