CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cyclooxigenase-2 mediates anandamide effect during implantation in the rat.
Autor/es:
SORDELLI MS; RIBEIRO ML; CAÑUMIL VA; FRANCHI AM; BELTRAME JS; MERESMAN G
Lugar:
Buenos Aires
Reunión:
Congreso; XX Jornadas anuales de la Sociedad Argentina de Biología, Buenos Aires, 5 al 7 de Diciembre 2018; 2018
Resumen:
Anandamide is a lipid mediator from the group of endocannabinoids that participates in embryo implantation. Anandamide tone is regulated by its degrading enzyme, fatty acid amide hydrolase (FAAH) and dysregulations in anandamide production are correlated with implantation failure and recurrent abortion. Also, cyclooxigenase-2 derived prostaglandins play a crucial role at implantation sites. During early pregnancy the endometrial stroma is mainly formed by fibroblasts, and later when embryo invasion begins, these fibroblasts develop into decidual cells. Yet, the role of stromal fibroblasts during early gestation remains unexplored. Also, it has been described that anandamide and prostaglandins regulate fibroblast behavior in many biological systems. Therefore, the aim of the present work was to investigate the crosstalk between anandamide and cyclooxigenase-2 in stromal fibroblasts during the implantation process. We adopted experimental designs in vitro and in vivo. First, Wistar females were sacrificed in day 5 of gestation, uterine horns were excised and incubated for 1 h with anandamide 1 nM alone or in the presence of indomethacin 1 µM (a non-selective cyclooxigenase-1 and 2 inhibitor) or meloxicam 1 µM (a selective cyclooxigenase-2 inhibitor). Day 5 pregnant rat uterus (day of implantation) is principally composed by stromal fibroblasts. We determined the production of prostaglandins by radioimmunoassay. For the in vivo approach, another group of rats received an intra-uterine injection of URB-597 in the left horn (a FAAH selective inhibitor, 2 µl, 1 mM) in day 5 of gestation. The right horn was injected with vehicle and considered as control. Parameters related to implantation events and cyclooxigenase-2 protein level (western blot) were studied in day 8 of pregnancy. We observed that incubation with anandamide increased the production of prostaglandins E2 (11.1 ± 0.8 vs 17.6 ± 0.6 pg/mg ph, p