CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A maternal diet enriched in saturated fat induces intestinal alterations related to metabolic pathologies in the fetuses and offspring
Autor/es:
GOMEZ RIBOT, DALMIRO; HEINECKE, MARÍA FLORENCIA; JAWERBAUM, ALICIA; FORNES, DAIANA; WHITE, VERÓNICA
Lugar:
CANCUN
Reunión:
Congreso; 6th International Symposium on Metabolic Programming and Microbiome. 3rd Meeting of Ibero-American DOHaD chapter; 2018
Institución organizadora:
Iberoamerican Chapter DOHAD
Resumen:
Maternal diets enriched in saturated fat program metabolic alterations in the offspring. This is considered a cause for the worldwide increase in obesity. The intestine plays an important role in metabolic regulation. It synthesizes incretins, regulates the absorption of nutrients and the excretion of endobiotic and xenobiotic substances and precludes commensalist microbiota and small-inflammatory substances from entering the organism, thus preventing systemic infection and inflammation. Moreover, intestine integrity failure due to alterations in the epithelial barrier causes low-grade inflammation to enter the porta system, affecting the liver. Therefore, a proper intestinal function is important to prevent metabolic alterations.Methods and Results The aim of our work was to evaluate intestinal expression of incretins and proteins involved in detoxifying function and epitelial barrier integrity in fetuses and offspring from rats fed with a saturated fat-enriched diet. Methods: Female Wistar rats were fed with standard or saturated fat diet (28% fat) since they were 6 week-old (SFD rats). After 8 weeks, they were mated with control males. Control and SFD rats were euthanized at 21 days of gestation or allowed to deliver and their offspring was euthanized at 140 days of age. The intestines from the fetuses and the offspring were obtained. mRNA levels of the incretin gastrointestinal peptide (GIP) and of the detoxifying channel multidrug resistance-associated proteins (MRP 2 and 3) were analyzed by RT-PCR. Protein levels and localization of Claudin-3 (Clau 3) were assessed by immunohistochemistry. We found a decrease in mRNA levels of GIP, MRP2 (20% p