CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Changes in the oocyte integrity and bone marrow induced by 3- methylcholanthrene and prevented by α-naphthoflavone
Autor/es:
GALARZA, ROCÍO ALEJANDRA; ZURITA, SILVIA; RHON-CALDERÓN, ERIC ALEJANDRO; FALETTI, ALICIA GRACIELA
Lugar:
Belgrade
Reunión:
Congreso; 10th congress of toxicology in developing countries (CTDC); 2018
Institución organizadora:
International union of toxicology
Resumen:
3-Methylcholanthrene (3MC) is an environmental pollutant that causes reproductive toxicity andgenotoxicity. Previous studies showed that 3MC alters the ovarian function by affecting the follicleintegrity, and causes DNA damage in peripheral blood, bone marrow and oocytes. Now, we studied the effect of daily exposure to 3MC on the oocyte integrity, induction of chromosome aberrations in bone marrow and the ability of α-naphthoflavone (αNF) to prevent this action. Immature rats were daily injected with a combination of 3MC (0.1 and 1 mg/kg) and αNF (80 mg/kg) for 20 days. Oocyte integrity, by morphological parameters; oocyte viability, by fluorescent dyes; and chromosome aberrations (CA) in bone marrow were evaluated. Compared with controls (C), both doses of 3MC increased the area (C 12.4±0.2 m2; 3MC 14.5±0.7 m2; P<0.001), perimeter (C 39±1 m; 3MC 48±2 m; P<0.001), and perivitelline space (C 1.6±0.1 m; 3MC 2.3±0.1 m; P<0.001); and decreased the thickness of the zona pellucida (C 1.7±0.1 m; 3MC 1.1±0.1 m; P<0.001) and the number of viable oocytes (C 86±3%; 3MC 60±3%; P<0.001). 3MC-treated rats exhibited a higher number of CA (C 7±2%; 3MC 20±4%; P<0.001), especially metaphases with dicentric chromosomes (C 2.4±0.5%; 3MC 8±2%; P<0.001) and fragments of chromosomes (C 1.0±0.5%; 3MC 3.6±0.4%; P<0.001). All these changes were prevented by daily treatment with αNF. In conclusion: i) daily exposure to 3MC alters the oocyte integrity; ii) low doses of 3MC are enough to induce CA in bone marrow; and iii) αNF prevents both the systemic and oocyte toxic effect of 3MC.