MATERNAL OVERWEIGHT ALTERS REPRODUCTIVE DEVELOPMENT IN MALE OFFSPRING BY MODIFYING TESTICULAR HISTOLOGY AND TESTOSTERONE LEVELS

BIZZOZZERO, MARIANNE; GALARZA, ROCÍO ALEJANDRA; CORTEZ, ANALÍA ELIZABETH; RHON-CALDERÓN, ERIC ALEJANDRO

Ciudad del Cabo

Congreso; 18th International Congress of Endocrinology; 2018

International Society of Endocrinology

An appropriate maternal body weight is needed for a normal fetal programming. Our previous studies showed that maternal overweight affects male offspring in: size and body weight, ano-genital distance, count, motility and morphology of the sperm. Now, we studied testicular histology and testosterone levels in male offspring from rats fed with standard (OSD) or cafeteria diet (OCD) from rats with 25% (OCD25) and 35% overweight (OCD35). Offspring were euthanized at 60 days of age and we analyzed: seminiferous tubular diameter (STD), thickness of the seminiferous epithelium (SE), tubular lumen diameter (TLD) (um); Leydig (LC), Sertoli (SC) and Spermatogonia (SgC) cells (cells/tubule); stages of the seminiferous tubules (percentage) and testosterone levels (ng/ml). Compared with OSD (STD: 59±2; SE: 15±1; TLD: 26±1; LC 61±1; SC 30±1; SgC 86±3), both OCD groups exhibited a decrease in parameters analyzed, but only significant for OCD35 (STD: 37±4, P