RESVERATROL EXERTS ANTI-INFLAMMATORY EFFECTS IN MICROGLIAL CELLS AND PROTECTS THE FETAL CENTRAL NERVOUS SYSTEM IN A MURINE MODEL OF MATERNAL INFLAMMATION

BARIANI MARÍA VICTORIA; FRANCHI ANA MARÍA; TICCHI JULIA; MESTRE LEYRE; GUAZA CARMEN; CORREA FERNANDO

Mar del Plata

Congreso; LXIII Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2018

SAIC

Introduction: Fetal exposure to an inflammatory environment during the central nervous system (CNS) development produces a series of structural, metabolic and epigenetic changes resulting in long-term neurological and neuropsychiatric consequences in adulthood. Therefore, pharmacological interventions aimed at preventing maternal inflammation could reduce these changes frequently observed in premature infants. Importantly, microglia plays a central role in the CNS innate immune response.Reveratrol, a polyphenol with anti-inflammatory and antioxidant properties, has demonstrated neuroprotective effects in several murine models.Objectives: To study the possible neuroprotective effects of resveratrol in the fetal CNS in a model of maternal inflammation induced by bacterial lipopolysaccharide (LPS) and to characterize the signaling pathways involved in the modulation of the microglial response.Experimental design: Resveratrol (30 mg/kg) was administered to Balb/c females on day 15 of pregnancy, exposed or not to two doses of LPS (0.17 mg/kg and 0.5 mg/kg, three hours apart). Five hours after the last administration of LPS, fetal brains were removed and frozen at -80°C until use. Additionally, primary cultures of microglial cells were carried out for in vitro signaling experiments.Results: Our results show that the LPS-triggered maternal inflammation induces the mRNA expression of the chemokines Rantes and Mcp-1 (p