Changes in the Oocyte Integrity and Bone Marrow Induced by 3-Methylcholanthrene and Prevented by α-Naphthoflavone

GALARZA, ROCÍO ALEJANDRA; RHON CALDERÓN ERIC ALEJANDRO; FALETTI, ALICIA GRACIELA; ZURITA, SILVIA

Belgrado

Congreso; 10th Congress of Toxicology in Developing Countries; 2018

CDTC

3-Methylcholanthrene (3MC) is an environmental pollutant that causes reproductive toxicity and genotoxicity. Previous studies showed that 3MC alters the ovarian function by affecting the follicle integrity, and causes DNA damage in peripheral blood, bone marrow and oocytes. Now, we studied the effect of daily exposure to 3MC on the oocyte integrity, induction of chromosome aberrations in bone marrow and the ability of α-naphthoflavone (αNF) to prevent this action. Immature rats were daily injected with a combination of 3MC (0.1 and 1 mg/kg) and αNF (80 mg/kg) for 20 days. Oocyte integrity, by morphological parameters; oocyte viability, by fluorescent dyes; and chromosome aberrations (CA) in bone marrow were evaluated. Compared with controls (C), both doses of 3MC increased the area (C 12.4±0.2 m2; 3MC 14.5±0.7 m2; P