CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Role of Reproductive Bioactive Lipids in Human First Trimester Trophoblast Migration.
Autor/es:
SORDELLI, MS; FRANCHI ANA M; BELTRAME, JS; CAÑUMIL, VA; MARKERT, UDO; RIBEIRO, MARÍA L.
Lugar:
Orlando, Florida
Reunión:
Congreso; 64th Annual Meeting of the Society for Reproductive Investigation (SRI); 2017
Resumen:
Role of ReproductiveBioactive Lipids in Human First Trimester Trophoblast Migration.Sordelli MS1, Beltrame JS1, Cañumil VA1, FranchiAM1, Markert UR2, Ribeiro ML1. 1CEFYBO,CONICET, Argentina. 2PlacentaLab, University Hospital Jena, Germany. Introduction: Bioactive lipids as lysophosphatidic acid(LPA) and prostaglandins (PGs) play major roles at the maternal-fetal interface.We reported that LPA augments cyclooxygenase-2 (COX-2) derived PGE2 synthesisin the rat uterus during implantation. Trophoblasts are the main source of LPA infirst trimester pregnancy, suggesting that LPA participates in its functions. PGE2regulates decidualization and vascularization, two processes parallel totrophoblast migration and invasion. Defects in these events are related toimplantation failures. Thus, we postulate that LPA and PGs crosstalk modulatecrucial events at the maternal-fetal interface. Our aim was to investigate LPAaction on human first trimester trophoblast migration and its interaction with PGE2. Methods: HTR-8/SVneo cell line was used as a model of human firsttrimester extravillous trophoblast. Cells were subjected to migration (woundhealing assay, 18h), invasion (transwell inserts of 8 µm coated with Matrigel,24h) and proliferation assays (MTS, 48h). PGE2 concentration was determined byradioimmunoassay and the localization of COX-1 and 2 by immunocytochemistry. Results: Treatment of HTR-8/SVneo with LPA (50 µM) augments trophoblastmigration and invasion (p